7KYS

Crystal structure of human BCCIP beta (Native2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2020-02-21
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	60.040, 67.000, 99.530
Unit cell angles	90.00, 91.03, 90.00

Refinement procedure

Resolution	29.730 - 2.200
R-factor	0.2053
Rwork	0.205
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kyq
RMSD bond length	0.009
RMSD bond angle	1.262
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	39873	2987
<I/σ(I)>	8.59	1.27
Completeness [%]	98.8
Redundancy	3.7
CC(1/2)	0.997	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M citric acid, pH 4.5, 1-2% PEG6000