7KYQ
Crystal structure of human BCCIP beta (Native1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 112.670, 112.670, 56.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.840 - 3.060 |
R-factor | 0.2191 |
Rwork | 0.217 |
R-free | 0.25070 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.539 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.090 |
High resolution limit [Å] | 3.040 | 3.040 |
Number of reflections | 7295 | 341 |
<I/σ(I)> | 12.4 | 2.3 |
Completeness [%] | 99.8 | |
Redundancy | 12.4 | |
CC(1/2) | 0.995 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M tri-sodium citrate, 18-20% PEG3350 |