7KYP
PsaBC from Streptococcus pneumoniae in complex with Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.550, 129.560, 169.620 |
| Unit cell angles | 95.35, 92.21, 98.66 |
Refinement procedure
| Resolution | 49.630 - 2.900 |
| R-factor | 0.26145 |
| Rwork | 0.260 |
| R-free | 0.28090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kyo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.712 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.080 | 0.697 |
| Number of reflections | 156113 | 15332 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 91.7 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES, pH 6.5, 0.2 M calcium chloride dihydrate, 33% v/v PEG400 |
