7KYM
Crystal structure of the MarR family transcriptional regulator from Bradyrhizobium japonicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.265, 62.125, 92.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.260 - 1.500 |
Rwork | 0.191 |
R-free | 0.20960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cjn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1-3660-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.260 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 44118 | 4182 |
<I/σ(I)> | 16.2 | |
Completeness [%] | 99.1 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris: HCl, pH 6.5, 20 % (w/v) PEG MME 5000 |