7KYL

Powassan virus Envelope protein DIII in complex with neutralizing Fab POWV-80

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-03-14
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 1 21 1
Unit cell lengths	79.154, 62.557, 141.546
Unit cell angles	90.00, 97.97, 90.00

Refinement procedure

Resolution	46.670 - 2.000
R-factor	0.191
Rwork	0.190
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svb 3uo1
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.116	0.532
Rmeas	0.125	0.577
Rpim	0.046	0.222
Total number of observations	666279	45594
Number of reflections	93018	6840
<I/σ(I)>	10.9	3.7
Completeness [%]	100.0	100
Redundancy	7.2	6.7
CC(1/2)	0.996	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293.15	0.16M ammonium sulfate, 0.08M sodium acetate, 20% w/v glycerol, 16% w/v PEG 4000