7KWM
CtBP1 (28-375) L182F/V185T - AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-10 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 89.262, 89.262, 163.658 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.160 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6v89 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.679 |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.160 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.108 | |
| Number of reflections | 17800 | 1723 |
| <I/σ(I)> | 17.65 | |
| Completeness [%] | 99.4 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.994 | 0.220 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM HEPES, pH 7.5, 140 mM calcium chloride, 4% PEG400 |
