7KV7
Surface glycan-binding protein B from Bacteroides fluxus in complex with laminaritriose
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 74.268, 59.249, 148.137 |
Unit cell angles | 90.00, 102.61, 90.00 |
Refinement procedure
Resolution | 35.664 - 1.760 |
Rwork | 0.159 |
R-free | 0.20180 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.642 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.380 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Number of reflections | 57885 | 2864 |
<I/σ(I)> | 21.6 | |
Completeness [%] | 93.5 | |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 0.1 M MES pH6.6, 0.2 M ammonium sulfate, 20% (w/v) PEG5000MME, 0.1 M guanidine hydrochloride |