7KV5

Surface glycan-binding protein B from Bacteroides fluxus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	59.533, 77.619, 146.621
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.709 - 1.820
Rwork	0.191
R-free	0.23010
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.555
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.710	1.860
High resolution limit [Å]	1.820	1.820
Number of reflections	30831	1532
<I/σ(I)>	12.3
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.1 M MES pH6.6, 0.2 M ammonium sulfate, 20 % (w/v) PEG5000MME 0.1 M guanidine hydrochloride