7KV2
Surface glycan-binding protein A from Bacteroides thetaiotaomicron
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.830, 71.685, 75.068 |
| Unit cell angles | 90.00, 105.10, 90.00 |
Refinement procedure
| Resolution | 38.260 - 1.800 |
| Rwork | 0.164 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.260 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 51588 | 2482 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.993 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M bis-tris pH6.5, 0.2M lithium sulfate, 25 % (w/v) PEG3350 |
