7KUP

2'-OMe modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2020-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977408
Spacegroup name	P 3 2 1
Unit cell lengths	48.972, 48.972, 82.204
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.440
Rwork	0.199
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uee
RMSD bond length	0.028
RMSD bond angle	3.208
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.460
High resolution limit [Å]	1.440	1.440
Rmerge	0.085	0.497
Rmeas	0.087	0.512
Rpim	0.020	0.122
Number of reflections	21287	1017
<I/σ(I)>	26.1	5.1
Completeness [%]	99.9	99.9
Redundancy	18.7	15.8
CC(1/2)	0.988	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate, 50 mM Magnesium chloride