7KUP
2'-OMe modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2020-06-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 48.972, 48.972, 82.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.440 |
| Rwork | 0.199 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uee |
| RMSD bond length | 0.028 |
| RMSD bond angle | 3.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.085 | 0.497 |
| Rmeas | 0.087 | 0.512 |
| Rpim | 0.020 | 0.122 |
| Number of reflections | 21287 | 1017 |
| <I/σ(I)> | 26.1 | 5.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18.7 | 15.8 |
| CC(1/2) | 0.988 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate, 50 mM Magnesium chloride |
