7KUO
FANA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2020-06-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977408 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 49.072, 49.072, 81.254 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.281 |
Rwork | 0.204 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uee |
RMSD bond length | 0.027 |
RMSD bond angle | 3.366 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.113 | 0.491 |
Rmeas | 0.117 | 0.504 |
Rpim | 0.114 | 0.027 |
Number of reflections | 5536 | 285 |
<I/σ(I)> | 29.2 | 7.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.8 | 19.2 |
CC(1/2) | 0.999 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.4 M Sodium malonate pH 7.0, 50 mM Magnesium chloride |