7KTL
DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Product State Ternary Complex, 50 mM Mn2+ (90min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.271, 62.099, 118.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.961 - 1.421 |
R-factor | 0.1592 |
Rwork | 0.158 |
R-free | 0.17380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.440 |
High resolution limit [Å] | 1.420 | 3.850 | 1.420 |
Rmerge | 0.069 | 0.058 | 0.884 |
Rmeas | 0.075 | 0.064 | 0.960 |
Rpim | 0.030 | 0.027 | 0.371 |
Total number of observations | 533551 | ||
Number of reflections | 84301 | 4412 | 4203 |
<I/σ(I)> | 12 | ||
Completeness [%] | 99.5 | 96.9 | 100 |
Redundancy | 6.3 | 5.6 | 6.6 |
CC(1/2) | 0.993 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |