7KTJ

DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Pre-Catalytic Ground State Ternary Complex, 20 mM Ca2+ (120min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.020, 62.099, 118.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.855 - 1.452
R-factor	0.1619
Rwork	0.161
R-free	0.18430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.480
High resolution limit [Å]	1.450	3.940	1.450
Rmerge	0.066	0.051	0.854
Rmeas	0.072	0.056	0.924
Rpim	0.028	0.022	0.347
Total number of observations	480860
Number of reflections	72011	3552	3558
<I/σ(I)>	11.6
Completeness [%]	91.6	84.1	92.3
Redundancy	6.7	6.4	6.7
CC(1/2)		0.994	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000