7KTA
DNA Polymerase Mu, 8-oxodGTP:Ct Pre-Catalytic Ground State Ternary Complex, 20 mM Ca2+ (120min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.905, 68.718, 110.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.441 - 1.844 |
R-factor | 0.1687 |
Rwork | 0.167 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.870 |
High resolution limit [Å] | 1.840 | 4.990 | 1.840 |
Rmerge | 0.083 | 0.080 | 0.935 |
Rmeas | 0.088 | 0.088 | |
Rpim | 0.034 | 0.035 | 0.398 |
Total number of observations | 287020 | ||
Number of reflections | 40039 | 2173 | 1984 |
<I/σ(I)> | 10.4 | ||
Completeness [%] | 100.0 | 99.3 | 99.9 |
Redundancy | 7.2 | 6.6 | 6.3 |
CC(1/2) | 0.992 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |