7KT8

DNA Polymerase Mu, 8-oxodGTP:At Product State Ternary Complex, 50 mM Mg2+ (180min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.891, 68.503, 110.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.763 - 1.701
R-factor	0.1693
Rwork	0.168
R-free	0.19120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.056	0.032	0.772
Rmeas	0.063	0.036	0.884
Rpim	0.028	0.016	0.419
Total number of observations	236218
Number of reflections	49270	2585	2399
<I/σ(I)>	12.6
Completeness [%]	97.1	94	97.3
Redundancy	4.8	5	4
CC(1/2)		0.999	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000