7KT7
DNA Polymerase Mu, 8-oxodGTP:At Reaction State Ternary Complex, 50 mM Mg2+ (60min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.908, 68.531, 110.589 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.951 - 1.760 |
R-factor | 0.1627 |
Rwork | 0.161 |
R-free | 0.18650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
Rmerge | 0.110 | 0.055 | 0.935 |
Rmeas | 0.123 | 0.062 | 1.055 |
Rpim | 0.054 | 0.026 | 0.480 |
Total number of observations | 233405 | ||
Number of reflections | 45715 | 2489 | 2241 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 99.7 | 99.8 | 99.9 |
Redundancy | 5.1 | 5.4 | 4.7 |
CC(1/2) | 0.994 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |