7KT4
DNA Polymerase Mu, 8-oxodGTP:At Reaction State Ternary Complex, 10 mM Mn2+ (30min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-11 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.119, 68.819, 110.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.432 - 1.922 |
R-factor | 0.1682 |
Rwork | 0.166 |
R-free | 0.20370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.960 |
High resolution limit [Å] | 1.922 | 5.240 | 1.930 |
Rmerge | 0.072 | 0.035 | 0.699 |
Number of reflections | 35481 | 1892 | 1729 |
<I/σ(I)> | 12.1 | ||
Completeness [%] | 99.8 | 96.9 | 99.7 |
Redundancy | 8.2 | 9.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |