7KT1
DNA Polymerase Mu, dGTP:At Reaction State Ternary Complex, 50 mM Mn2+ (180min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.754, 68.793, 110.223 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.011 - 1.665 |
R-factor | 0.1658 |
Rwork | 0.165 |
R-free | 0.18860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.293 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
High resolution limit [Å] | 1.665 | 4.530 | 1.670 |
Rmerge | 0.093 | 0.092 | 0.895 |
Rmeas | 0.100 | 0.099 | 0.987 |
Rpim | 0.038 | 0.037 | 0.408 |
Number of reflections | 53801 | 2922 | 2600 |
<I/σ(I)> | 11.6 | ||
Completeness [%] | 99.8 | 99.9 | 99.3 |
Redundancy | 7.1 | 7.1 | 5.7 |
CC(1/2) | 0.981 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG 4000 |