7KSZ
DNA Polymerase Mu, dGTP:At Pre-Catalytic Ground State Ternary Complex, 10 mM Ca2+ (960min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.753, 68.778, 110.133 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.826 - 1.417 |
R-factor | 0.1582 |
Rwork | 0.157 |
R-free | 0.17470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.197 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.440 |
High resolution limit [Å] | 1.417 | 3.850 | 1.420 |
Rmerge | 0.097 | 0.081 | 0.919 |
Rmeas | 0.108 | 0.091 | 1.021 |
Rpim | 0.046 | 0.040 | 0.440 |
Number of reflections | 86531 | 4587 | 4279 |
<I/σ(I)> | 10.6 | ||
Completeness [%] | 99.8 | 98.8 | 100 |
Redundancy | 5.5 | 5.1 | 5.1 |
CC(1/2) | 0.986 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG 4000 |