7KSY
DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mg2+ (960min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-15 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.316, 62.323, 119.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.037 - 1.577 |
R-factor | 0.1565 |
Rwork | 0.156 |
R-free | 0.16950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.067 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
High resolution limit [Å] | 1.577 | 4.290 | 1.580 |
Rmerge | 0.095 | 0.092 | 0.569 |
Rmeas | 0.104 | 0.101 | 0.638 |
Rpim | 0.040 | 0.040 | 0.283 |
Number of reflections | 62385 | 3357 | 3095 |
<I/σ(I)> | 11 | ||
Completeness [%] | 99.9 | 99.4 | 100 |
Redundancy | 6.4 | 6.2 | 4.7 |
CC(1/2) | 0.989 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |