7KSX
DNA Polymerase Mu, dGTP:Ct Product State Ternary Complex, 10 mM Mg2+ (30min)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.011, 68.695, 110.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.832 - 1.570 |
| R-factor | 0.1645 |
| Rwork | 0.163 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m04 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2-3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 4.260 | 1.570 |
| Rmerge | 0.046 | 0.027 | 0.844 |
| Rmeas | 0.051 | 0.031 | 0.941 |
| Rpim | 0.022 | 0.014 | 0.409 |
| Total number of observations | 338599 | ||
| Number of reflections | 64263 | 3452 | 3146 |
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 99.7 | 99.1 | 99.5 |
| Redundancy | 5.3 | 5 | 5 |
| CC(1/2) | 0.998 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |
