7KSW

DNA Polymerase Mu, dGTP:Ct Reaction State Ternary Complex, 10 mM Mg2+ (10min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-17
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.043, 68.736, 110.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.490
R-factor	0.1553
Rwork	0.154
R-free	0.17180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.013
RMSD bond angle	1.312
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.520
High resolution limit [Å]	1.490	4.040	1.490
Rmerge	0.093	0.100	0.930
Rmeas	0.100	0.108	0.999
Rpim	0.036	0.040	0.359
Total number of observations	563041
Number of reflections	75406	4044	3718
<I/σ(I)>	11.1
Completeness [%]	99.8	99.7	100
Redundancy	7.5	7	7.6
CC(1/2)		0.981	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000