7KST

DNA Polymerase Mu, dGTP:Ct Reaction State Ternary Complex, 10 mM Mn2+ (2min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.865, 68.831, 110.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.788 - 1.599
R-factor	0.1646
Rwork	0.164
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.011
RMSD bond angle	1.220
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.599	4.340	1.600
Rmerge	0.078	0.051	0.928
Rmeas	0.086	0.056	1.025
Rpim	0.036	0.023	0.430
Number of reflections	59911	3172	2960
<I/σ(I)>	10.7
Completeness [%]	98.3	96.6	98.3
Redundancy	5.8	5.7	5.4
CC(1/2)		0.995	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000