7KSS
DNA Polymerase Mu, dGTP:Ct Pre-Catalytic Ground State Ternary Complex, 10 mM Ca2+ (20min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-08-17 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.023, 68.767, 110.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.839 - 1.503 |
R-factor | 0.1607 |
Rwork | 0.160 |
R-free | 0.17690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.189 | 0.213 | 0.937 |
Rmeas | 0.203 | 0.229 | 1.045 |
Rpim | 0.073 | 0.083 | 0.456 |
Total number of observations | 497661 | ||
Number of reflections | 71579 | 3724 | 3542 |
<I/σ(I)> | 9 | ||
Completeness [%] | 97.8 | 94.6 | 97.4 |
Redundancy | 7 | 6.8 | 5.1 |
CC(1/2) | 0.955 | 0.645 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |