7KSF

Crystal structure of Prototype Foamy Virus Protease-Reverse Transcriptase (native)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97601
Spacegroup name	C 1 2 1
Unit cell lengths	228.210, 52.540, 75.430
Unit cell angles	90.00, 90.09, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.2439
Rwork	0.225
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kse
RMSD bond length	0.004
RMSD bond angle	0.714
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.128	1.532
Rmeas	0.157	1.888
Rpim	0.089	1.089
Number of reflections	19435	2867
<I/σ(I)>	7.1	1
Completeness [%]	96.1	88.4
Redundancy	5.1	5.1
CC(1/2)	0.993	0.334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	50 mM KCl, 50 mM sodium cacodylate trihydrate pH 6.0, 12% PEG 8000, 1.0 mM spermine, 1.0 mM L-argininamide, 200 mM glycyglycine or glycylglycylglycine, 100 mM CaCl2