7KS4
Thermus Phage P74-26 Large Terminase ATPase domain with partially bound ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 129.515, 129.515, 129.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.790 - 1.887 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.22110 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.933 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 1.960 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.063 | 1.000 |
| Number of reflections | 29147 | 2900 |
| <I/σ(I)> | 21.5 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 100 mM Sodium Acetate:HCl pH 5.0, 0.5M ammonium sulfate. |
