7KRW

Stimulating state of near full-length Hsp70 DnaK fused with a substrate peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 3
Unit cell lengths	294.203, 294.203, 294.203
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.630 - 7.700
R-factor	0.4549
Rwork	0.304
R-free	0.31640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kru
RMSD bond length	0.009
RMSD bond angle	1.371
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.630	39.380	7.980
High resolution limit [Å]	7.700	15.660	7.700
Rmerge	0.632	0.116	3.700
Rmeas	0.644	0.123	3.770
Rpim	0.121	0.041	0.740
Number of reflections	5107	594	510
<I/σ(I)>	8.97		0.56
Completeness [%]	97.1	92.5	99.2
Redundancy	26.7	7.8	25.5
CC(1/2)	0.947	0.989	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M HEPES pH 7, 3.2 M ammonium sulfate