7KRT

Restraining state of a truncated Hsp70 DnaK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	77.469, 199.529, 94.231
Unit cell angles	90.00, 93.75, 90.00

Refinement procedure

Resolution	47.020 - 2.790
R-factor	0.2796
Rwork	0.279
R-free	0.30830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ko2
RMSD bond length	0.004
RMSD bond angle	0.694
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.020	2.890
High resolution limit [Å]	2.790	2.790
Rmerge	0.713	3.250
Rmeas	0.739	3.380
Rpim	0.195	0.910
Number of reflections	70764	7064
<I/σ(I)>	5.74
Completeness [%]	99.6	99.3
Redundancy	14.2	13.6
CC(1/2)	0.784	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2 M magnesium acetate, 0.1 M sodium cacodylate pH 6.5, 20% PEG 8000