7KR6
Glycoside hydrolase family 16 endo-glucanase from Bacteroides ovatus in complex with G4G3G-2F-DNP
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.447, 84.576, 67.105 |
Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
Resolution | 39.400 - 1.560 |
Rwork | 0.172 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nbo |
RMSD bond length | 0.012 |
RMSD bond angle | 1.707 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.400 | 1.590 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 143215 | 6822 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 98.7 | |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.935 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.2 M sodium chloride, 0.1 M Tris pH 8.5, 25% (w/v) PEG3350 |