7KPZ

1.70 A resolution crystal structure of Group A Streptococcus HupZ-V5-His6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97913
Spacegroup name	P 21 21 21
Unit cell lengths	40.020, 62.378, 94.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.761 - 1.703
R-factor	0.1863
Rwork	0.184
R-free	0.21870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5esc
RMSD bond length	0.007
RMSD bond angle	1.056
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.059	0.049	0.183
Rmeas	0.062	0.052	0.193
Rpim	0.020	0.017	0.061
Total number of observations	258352
Number of reflections	26686	1470	1332
<I/σ(I)>	10.8
Completeness [%]	99.7	99.1	99.9
Redundancy	9.7	9.1	9.9
CC(1/2)		0.998	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	295	20% PEG3350, 0.2 M lithium acetate