7KPT
Crystal structure of CtdE in complex with FAD and substrate 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.279, 48.527, 67.643 |
| Unit cell angles | 90.00, 114.49, 90.00 |
Refinement procedure
| Resolution | 34.020 - 1.910 |
| R-factor | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kpq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc3_4028) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.849 |
| Rmeas | 0.088 | 0.949 |
| Rpim | 0.040 | 0.421 |
| Number of reflections | 35699 | 1774 |
| <I/σ(I)> | 26.2 | 6 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.811 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Calcium chloride, 20% w/v PEG 6000, 10% v/v Ethylene glycol |
