7KPO
High Resolution Crystal Structure of the DNA-binding Domain from the Sensor Histidine Kinase ChiS from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.912, 78.606, 72.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.970 - 1.280 |
R-factor | 0.146 |
Rwork | 0.144 |
R-free | 0.18030 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.217 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.076 | 0.757 |
Rmeas | 0.083 | 0.854 |
Rpim | 0.032 | 0.386 |
Number of reflections | 38590 | 1866 |
<I/σ(I)> | 28.3 | 2.3 |
Completeness [%] | 99.7 | 97.3 |
Redundancy | 6.9 | 4.6 |
CC(1/2) | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | Protein: 6.3 mg/ml, 0.01M Tris pH 8.3; Screen: Classics II (G2), 0.2M Lithium sulfate, 0.1M Bis-Tris pH 5.5, 25% (w/v) PEG 3350 |