7KPM

Crystal structure of hEphB1 bound with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	33.134, 91.362, 96.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.455 - 1.608
R-factor	0.1685
Rwork	0.167
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zfx
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.250	1.550
Number of reflections	38492	3783
<I/σ(I)>	16.5	2.8
Completeness [%]	99.1
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M sodium malonate, pH 4.6, 14% PEG3350