7KO2
Restraining state of near full-length Hsp70 DnaK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.431, 77.490, 182.741 |
Unit cell angles | 90.00, 101.79, 90.00 |
Refinement procedure
Resolution | 49.540 - 2.640 |
R-factor | 0.242 |
Rwork | 0.241 |
R-free | 0.28660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b9q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.678 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.540 | 2.730 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.554 | 2.420 |
Rmeas | 0.565 | 2.460 |
Rpim | 0.108 | 0.460 |
Number of reflections | 81891 | 8136 |
<I/σ(I)> | 9.88 | 0.69 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 27.9 | 28.5 |
CC(1/2) | 0.938 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M sodium cacodylate pH 6.5, 30% PEG 8000, 0.2 M sodium acetate |