7KMP

Crystal structure of the GH31 alpha-xylosidase (Xac1773) from Xanthomonas citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.4587
Spacegroup name	P 21 21 2
Unit cell lengths	117.450, 146.410, 63.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.807 - 1.556
R-factor	0.1628
Rwork	0.162
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xvg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3139)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.807	1.640
High resolution limit [Å]	1.550	1.550
Number of reflections	155571	24655
<I/σ(I)>	20.15	6.96
Completeness [%]	99.7	98.7
Redundancy	9.71
CC(1/2)	0.990	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 mol/L potassium nitrate 20% PEG 3350