7KM4
Crystal Structure of Oxidized Version of Redox-Sensitive Superfolder Green Fluorescent Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2017-08-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.735, 68.735, 99.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.430 - 2.650 |
R-factor | 0.2394 |
Rwork | 0.230 |
R-free | 0.32080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.277 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rpim | 0.031 | 0.249 |
Number of reflections | 7433 | 1426 |
<I/σ(I)> | 3.3 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.3 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 200, Potassium Chloride, 20% PEG-3350 |