7KLF
Ternary structure of Dpo4 bound to G in the template base paired with incoming dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 99.520, 102.373, 52.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.538 - 2.301 |
R-factor | 0.2335 |
Rwork | 0.231 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qz7 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.628 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 10.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.180 | 2.300 |
Rmerge | 0.091 | 0.035 | 0.725 |
Total number of observations | 263794 | ||
Number of reflections | 24689 | 1360 | 1201 |
<I/σ(I)> | 8.1 | ||
Completeness [%] | 100.0 | 99.9 | 99.9 |
Redundancy | 10.7 | 10.1 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12 to 18% PEG 3350, 100 mM HEPES (pH 6.6),100 mM calcicum acetate, and 2.5% glycerol |