7KJI

Plasmodium falciparum protein Pf12p bound to nanobody D9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953649
Spacegroup name	P 21 21 21
Unit cell lengths	61.991, 67.098, 110.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.753 - 3.250
R-factor	0.2534
Rwork	0.245
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kj7
RMSD bond length	0.003
RMSD bond angle	0.578
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.753	42.753	3.440
High resolution limit [Å]	3.250	9.550	3.250
Rmerge	0.153	0.048	2.018
Rmeas	0.160	0.050	2.100
Total number of observations	101019
Number of reflections	7710	346	1207
<I/σ(I)>	13.51	44.77	1.44
Completeness [%]	99.8	99.1	99.3
Redundancy	13.102	10.968	12.978
CC(1/2)	0.999	0.999	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	0.1 M bis-tris chloride pH 6.5, 0.2 M magnesium chloride, 25% PEG3350