7KJ6
Structure of Legionella Effector LegA15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.160, 75.050, 108.530 |
| Unit cell angles | 90.00, 99.46, 90.00 |
Refinement procedure
| Resolution | 47.940 - 2.500 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.23680 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.745 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.940 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.454 |
| Number of reflections | 67586 | 4999 |
| <I/σ(I)> | 11.02 | |
| Completeness [%] | 99.3 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Tris pH 8.5, 20% Glycerol, 2% acetonitrile and 22% PEG1500 |
