7KG7

Structure of human PARG complexed with PARG-292

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	66.607, 89.038, 94.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.900 - 1.850
R-factor	0.1783
Rwork	0.177
R-free	0.19550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b1g
RMSD bond length	0.005
RMSD bond angle	0.715
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.900	1.916
High resolution limit [Å]	1.850	1.850
Rmerge	0.092	1.356
Rmeas	0.097	1.428
Rpim	0.029	0.440
Number of reflections	48514	4759
<I/σ(I)>	14.26	1.83
Completeness [%]	99.3	99.31
Redundancy	10.5	10.3
CC(1/2)	0.999	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M PCTP, pH 7.5, 0.2 M AmSO4, 18-23% PEG3350