7KE5

Heavy chain ferritin with N-terminal EBNA1 epitope

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 42 21 2
Unit cell lengths	219.323, 219.323, 148.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.820 - 2.800
R-factor	0.2765
Rwork	0.274
R-free	0.31650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j4a
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.820	48.820	2.850
High resolution limit [Å]	2.800	14.820	2.800
Rmerge	0.252	0.078	0.898
Rmeas	0.259	0.080	0.928
Rpim	0.056	0.017	0.228
Total number of observations	1766865	16527	66508
Number of reflections	85483	686	4260
<I/σ(I)>	7.9	20.3	2.6
Completeness [%]	95.9	96.8	91.6
Redundancy	20.7	24.1	15.6
CC(1/2)	0.998	0.997	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.1M ammonium citrate tribasic pH 7.0, 12% PEG 3350