7KE1
Factor H enhancing human antibody fragment (Fab) to meningococcal Factor H binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11583 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.771, 97.260, 70.472 |
Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
Resolution | 70.470 - 1.500 |
R-factor | 0.1623 |
Rwork | 0.162 |
R-free | 0.18260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
RMSD bond length | 0.011 |
RMSD bond angle | 1.265 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.470 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.167 | 1.012 |
Rmeas | 0.180 | 1.086 |
Rpim | 0.067 | 0.394 |
Number of reflections | 143102 | 97061 |
<I/σ(I)> | 12.18 | 1.89 |
Completeness [%] | 98.1 | 96.35 |
Redundancy | 6.9 | 7 |
CC(1/2) | 0.998 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 3350, Bis-Tris Propane, lithium sulfate |