7KD4
Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329 -388), fused to MBP. Space group P21.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2016-10-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.561, 70.074, 111.739 |
Unit cell angles | 90.00, 96.47, 90.00 |
Refinement procedure
Resolution | 59.329 - 1.312 |
Rwork | 0.150 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kyc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.454 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230 2018/03/05) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.330 | 1.330 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmerge | 0.051 | 0.607 |
Rmeas | 0.061 | 0.857 |
Rpim | 0.032 | 0.605 |
Number of reflections | 149703 | 307 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 70.7 | 2.9 |
Redundancy | 3.2 | 1.5 |
CC(1/2) | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 1.65 M Ammonium sulphate, 0.2M Malic acid/KOH pH 5.5, Crystals were transferred into the following cryo-protective solution before vitrification: 1.65 M Ammonium sulphate, 0.2M Malic acid/KOH pH 5.5, 5mM Maltose, 1 M Lithium sulfate |