7KD3
Structure of an HxlR/DUF24 family transcription regulator, CdTR_3200 from hypervirulent Clostridioides difficile R20291
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.019, 55.001, 72.317 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 29.500 - 2.300 |
| R-factor | 0.2062 |
| Rwork | 0.200 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hzt |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.016 | 0.121 |
| Rmeas | 0.023 | 0.171 |
| Rpim | 0.016 | 0.121 |
| Number of reflections | 10495 | 1063 |
| <I/σ(I)> | 17.3 | 4 |
| Completeness [%] | 96.4 | 97.34 |
| Redundancy | 2 | 1.99 |
| CC(1/2) | 0.999 | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 0.2 M di-ammonium citrate pH 5.0, 20% PEG 3350 |
