7KD2
Ricin bound to VHH antibody V11B2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-09 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.607, 99.559, 111.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.189 - 2.550 |
R-factor | 0.2292 |
Rwork | 0.227 |
R-free | 0.28020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aai 3ezj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.081 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.546 | 6.920 | 2.550 |
Rmerge | 0.211 | 0.061 | 1.709 |
Rmeas | 0.230 | 0.066 | 1.895 |
Rpim | 0.091 | 0.026 | 0.807 |
Number of reflections | 28832 | 1557 | 1425 |
<I/σ(I)> | 3.7 | ||
Completeness [%] | 99.7 | 97.6 | 99.6 |
Redundancy | 6.2 | 6.4 | 5.4 |
CC(1/2) | 0.998 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 200 mM Zinc Acetate, 100 mM MES pH 6.0, 10% PEG 8,000 |