7KD0

Ricin bound to VHH antibody V2C11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-22
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	76.223, 100.817, 129.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.428 - 2.768
R-factor	0.2095
Rwork	0.207
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aai 3ezj
RMSD bond length	0.003
RMSD bond angle	0.599
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.820
High resolution limit [Å]	2.768	7.510	2.770
Rmerge	0.179	0.068	0.816
Rmeas	0.198	0.075	0.985
Rpim	0.081	0.030	0.541
Number of reflections	26051	1422	1242
<I/σ(I)>	4.7
Completeness [%]	99.5	98.5	95.3
Redundancy	5.5	6	2.9
CC(1/2)		0.996	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM Zinc Acetate, 14% PEG 8,000, 22% Glycerol, 80 mM NaCaco pH 6.5, 3% D+Galactose