7KC8
Salivary protein from Culex quinquefasciatus that belongs to the Cysteins and Tryptophan-Rich (CWRC) family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2020-02-26 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.541800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.940, 73.050, 106.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.740 - 2.300 |
R-factor | 0.2087 |
Rwork | 0.205 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.740 | 26.740 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.031 | 0.021 | 0.201 |
Rmeas | 0.032 | 0.022 | 0.215 |
Number of reflections | 25008 | 331 | 1367 |
<I/σ(I)> | 59.38 | 103.53 | 8.92 |
Completeness [%] | 96.1 | 97.1 | 71.8 |
Redundancy | 11.458 | 9.417 | 7.963 |
CC(1/2) | 1.000 | 1.000 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Sodium acetate trihydrate, 0.1M TRIS hydrochloride pH 8.5, 30% w/v PEG 4000 |