7KBM
Reverse Transcriptase Diabody with R83C Mutation Crystallized in C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.638, 86.302, 36.249 |
Unit cell angles | 90.00, 116.50, 90.00 |
Refinement procedure
Resolution | 33.380 - 2.000 |
R-factor | 0.2114 |
Rwork | 0.209 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vrp |
RMSD bond length | 0.002 |
RMSD bond angle | 0.536 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (v1.14-3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.101 | 0.347 |
Rmeas | 0.120 | 0.417 |
Number of reflections | 13604 | 669 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 98.3 | 97.5 |
Redundancy | 3.4 | |
CC(1/2) | 0.983 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M Sodium acetate trihydrate pH 4.5, 30% PEG 300 |