7KBM

Reverse Transcriptase Diabody with R83C Mutation Crystallized in C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	73.638, 86.302, 36.249
Unit cell angles	90.00, 116.50, 90.00

Refinement procedure

Resolution	33.380 - 2.000
R-factor	0.2114
Rwork	0.209
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vrp
RMSD bond length	0.002
RMSD bond angle	0.536
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (v1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.101	0.347
Rmeas	0.120	0.417
Number of reflections	13604	669
<I/σ(I)>	11.6
Completeness [%]	98.3	97.5
Redundancy	3.4
CC(1/2)	0.983	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Sodium acetate trihydrate pH 4.5, 30% PEG 300