7KBL
Biotin Carboxylase domain of Thermophilic 2-Oxoglutarate Carboxylase bound to Bicarbonate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.496, 125.154, 125.949 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.746 - 2.300 |
R-factor | 0.1833 |
Rwork | 0.181 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 1ULZ |
RMSD bond length | 0.013 |
RMSD bond angle | 1.158 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.240 | 2.240 |
Number of reflections | 51900 | 2693 |
<I/σ(I)> | 20.8 | |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 6.9 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 3350, 0.02M Citric Acid, 0.08M Bis-Tris-Propane |