7K9E
Crystal structure of Bacillus halodurans OapB in complex with its OLE RNA target (crystal form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-31 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 65 |
Unit cell lengths | 71.990, 71.990, 223.905 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.345 - 2.100 |
R-factor | 0.1734 |
Rwork | 0.172 |
R-free | 0.20400 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.370 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.048 | 0.926 |
Rmeas | 0.057 | 1.087 |
Rpim | 0.030 | 0.565 |
Number of reflections | 37999 | 3797 |
<I/σ(I)> | 17.2 | 1.2 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.998 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 240 mM sodium malonate (pH 6.0 to 7.0) and 20 to 21% PEG 3350 |